D-galactose transport in rat intestinal brush border membrane vesicles studied with a molecular-sieve technique.
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چکیده
منابع مشابه
The intestinal transport of zinc studied using brush-border-membrane vesicles from the piglet.
Brush-border-membrane vesicles were prepared from piglet small intestines and the uptake of 65Zn was studied using a rapid filtration assay. The mechanism of 65Zn uptake was complex and two processes were identified. In the first process, 65Zn uptake was rapid, reached equilibrium in 5-15 min and had an optimum pH of 7.5. The uptake was saturable and involved both binding to, and transport acro...
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Transport of glutamine by brush-border vesicles prepared from the renal cortex was studied. The transport system had both Na+-dependent and Na+-independent components. The presence of Na+ in the incubation resulted in an 'overshoot' at 30s at which time the rates of transport were approx. 8 times the values obtained in the absence of Na+. Variation of the glutamine concentration showed that the...
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D-Glucose transport was investigated in isolated brush-border membrane vesicles from human small intestine. Characteristics of D-glucose transport from the jejunum were compared with that in the mid and terminal ileum. Jejunal and mid-ileal D-glucose transport was Na+-dependent and electrogenic. The transient overshoot of jejunal D-glucose transport was significantly greater than corresponding ...
متن کاملbrush-border membrane vesicles
The characteristics of hypoxanthine transport were examined in purified brush-border membrane vesicles isolated from guineapig kidney. Hypoxanthine uptake in the vesicles was specifically stimulated by both Na+ and an inside-negative potential, resulting in a transient accumulation of intravesicular hypoxanthine. Na+-dependent hypoxanthine influx was saturable (apparent Km 4.4 + 2.1 ,uM, Vmax 1...
متن کاملRat Intestinal Brush Border Membrane Peptidases
The properties of two purified peptidases derived from the intestinal brush border membrane of the rat have been investigated. The pH optima, heat stabilities, substrate specificities, and metal ion requirements of the two enzymes and the effects of inhibitors on their activities were nearly identical. The isoenzymes catalyzed the hydrolysis of a wide range of peptides containing from 2 to 8 am...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1986
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1986.sp016106